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主营:分子类,蛋白类,抗体类,生化类试剂
℡ 4000-520-616
℡ 4000-520-616
Uscnk/ELISA Kit for Ciliary Neurotrophic Factor (CNTF)/96T*100/SEA021Hu
产品编号:SEA021Hu
市  场 价:¥655200.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$32760.00
品      牌: Uscnk
公      司:Uscnk
公司分类:
Uscnk/ELISA Kit for Ciliary Neurotrophic Factor (CNTF)/96T*100/SEA021Hu
商品介绍

ELISA Kit for Ciliary Neurotrophic Factor (CNTF)

HCNTF

  • Cytokine
  • Neuro science
  • Featured-Product
  • Product No.SEA021Hu
  • Organism SpeciesHomo sapiens (Human) Same name, Different species.
    • All
    • Human
    • Mouse
    • Rat
    • Cavia
    • Rabbit
    • Simian
    • Caprine
    • Ovine
    • Equine
    • Bovine
    • Porcine
    • Gallus
    • Canine
    • Others
    • Multi-species
    • Pan-species
  • Test MethodDouble-antibody Sandwich
  • Assay Length3h
  • Detection Range7.8-500pg/mL
  • SensitivityThe minimum detectable dose of this kit is typically less than 2.7pg/mL.
  • Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • DownloadInstruction Manual
  • UOM48T96T96T*596T*1096T*100
  • FOBUS$ 328 For more details, please contact local distributors!US$ 468 For more details, please contact local distributors!US$ 2106 For more details, please contact local distributors!US$ 3978 For more details, please contact local distributors!US$ 32760 For more details, please contact local distributors!
  • ELISA Kit for Ciliary Neurotrophic Factor (CNTF)Packages (Simulation)
  • ELISA Kit for Ciliary Neurotrophic Factor (CNTF)Packages (Simulation)
  • ELISA Kit for Ciliary Neurotrophic Factor (CNTF)Results demonstration
  • SEA021Hu.jpgTypical Standard Curve
  • CertificateISO9001: 2008, ISO13485: 2003 Registered

Specificity of the ELISA Kit for Ciliary Neurotrophic Factor (CNTF)

This assay has high sensitivity and excellent specificity for detection of Ciliary Neurotrophic Factor (CNTF).No significant cross-reactivity or interference between Ciliary Neurotrophic Factor (CNTF) and analogues was observed.

Recovery of the ELISA Kit for Ciliary Neurotrophic Factor (CNTF)

Matrices listed below were spiked with certain level of recombinant Ciliary Neurotrophic Factor (CNTF) and the recovery rates were calculated by comparing the measured value to the expected amount of Ciliary Neurotrophic Factor (CNTF) in samples.

MatrixRecovery range (%)Average(%)
serum(n=5)79-8883
EDTA plasma(n=5)80-9587
heparin plasma(n=5)86-10299

Precision of the ELISA Kit for Ciliary Neurotrophic Factor (CNTF)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ciliary Neurotrophic Factor (CNTF) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ciliary Neurotrophic Factor (CNTF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10%>Inter-Assay: CV<12%>

Linearity of the ELISA Kit for Ciliary Neurotrophic Factor (CNTF)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Ciliary Neurotrophic Factor (CNTF) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)92-101%79-96%78-93%78-103%
EDTA plasma(n=5)80-102%97-105%83-101%87-104%
heparin plasma(n=5)79-92%97-105%97-104%87-101%

Stability of the ELISA Kit for Ciliary Neurotrophic Factor (CNTF)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the ELISA Kit for Ciliary Neurotrophic Factor (CNTF)

1. Prepare all reagents, samples and standards;2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;4. Aspirate and wash 3 times;5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;6. Aspirate and wash 5 times;7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;8. Add 50µL Stop Solution. Read at 450nm immediately.

Test principle of the ELISA Kit for Ciliary Neurotrophic Factor (CNTF)

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ciliary Neurotrophic Factor (CNTF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ciliary Neurotrophic Factor (CNTF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ciliary Neurotrophic Factor (CNTF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ciliary Neurotrophic Factor (CNTF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

GIVEAWAYS

ELISA / CLIA Experiment Service

INCREMENT SERVICES

Single-component Reagents of Assay KitLysis Buffer Specific for ELISA / CLIAQuality Control of ELISA / CLIAELISA Kit Customized ServiceDisease Model Customized ServiceSerums Customized ServiceTGFB1 Activation ReagentReal Time PCR Experimental ServiceStreptavidin

Related products

Catalog No.Organism species: Homo sapiens (Human) Applications (RESEARCH USE ONLY!)
APA021Hu01Active Ciliary Neurotrophic Factor (CNTF)Cell culture; Activity Assays.
RPA021Hu01Recombinant Ciliary Neurotrophic Factor (CNTF)Positive Control; Immunogen; SDS-PAGE; WB.
PAA021Hu01Polyclonal Antibody to Ciliary Neurotrophic Factor (CNTF)WB; IHC; ICC; IP.
LAA021Hu71Biotin-Linked Polyclonal Antibody to Ciliary Neurotrophic Factor (CNTF)WB; IHC; ICC.
MAA021Hu22Monoclonal Antibody to Ciliary Neurotrophic Factor (CNTF)WB; IHC; ICC; IP.
SEA021HuELISA Kit for Ciliary Neurotrophic Factor (CNTF)Enzyme-linked immunosorbent assay for Antigen Detection.
SCA021HuCLIA Kit for Ciliary Neurotrophic Factor (CNTF)Chemiluminescent immunoassay for Antigen Detection.
GUESTBOOK
品牌介绍

Uscnk重组胃泌素(GT)

GAST;加油站; 胃泌素成分I;大胃泌素;胃泌素成分II; 胃泌素成分III

  • 产品编号

  • RPB224Mu01

  • 生物物种小家鼠(Mouse musculus)(鼠)同名,不同种。

  • 资源原核表达

  • 主办大肠杆菌

  • 内毒素水平每1µg <1.0EU(通过LAL方法确定)

  • 亚细胞定位秘密的

  • 预测分子量42.6kDa的

  • 准确的分子质量43kDa(差异分析请参见手册)

  • 残基和标签Net His和GST标签的Met1〜Gln101

  • 缓冲液配方20mM Tris,150mM NaCl,pH8.0,包含1mM EDTA,1mM DTT,0.01%SKL,5%海藻糖和Proclin300。

  • 特质冻干粉

  • 纯度> 90%

  • 等电点6.3

  • 应用领域积极控制;免疫原 SDS-PAGE;WB。
    如果需要蛋白质的生物活性,请检查活性蛋白质。

  • 下载使用说明书

  • 单位10微克为50μg 200μg的 1mg的 5毫克

  • 离岸价US $ 480



重组胃泌素(GT)的序列




重组胃泌素(GT)的用途

在20mM Tris,150mM NaCl(pH8.0)中复溶至0.1-1.0 mg / mL的浓度。不要涡旋。

重组胃泌素(GT)的存储

避免重复冷冻/解冻循环。在2-8°C下保存一个月。分装并在-80°C下存储12个月。

重组胃泌素(GT)的稳定性

热稳定性由损失率描述。损失率通过加速热降解试验确定,即将蛋白质在37°C下孵育48h,没有观察到明显的降解和沉淀。在适当的存储条件下,有效期内的丢失率小于5%。

礼物

聚丙烯酰胺凝胶电泳(PAGE)实验服务


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