This assay has high sensitivity and excellent specificity for detection of Transforming Growth Factor Beta 2 (TGFb2).No significant cross-reactivity or interference between Transforming Growth Factor Beta 2 (TGFb2) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Transforming Growth Factor Beta 2 (TGFb2) and the recovery rates were calculated by comparing the measured value to the expected amount of Transforming Growth Factor Beta 2 (TGFb2) in samples.
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 84-98 | 95 |
EDTA plasma(n=5) | 84-92 | 87 |
heparin plasma(n=5) | 93-103 | 98 |
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Transforming Growth Factor Beta 2 (TGFb2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transforming Growth Factor Beta 2 (TGFb2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10%>10%>Inter-Assay: CV<12%>12%>
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Transforming Growth Factor Beta 2 (TGFb2) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 80-88% | 92-101% | 82-94% | 81-98% |
EDTA plasma(n=5) | 90-98% | 81-103% | 96-104% | 97-105% |
heparin plasma(n=5) | 97-105% | 91-102% | 90-104% | 82-91% |
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
1. Prepare all reagents, samples and standards;2. Add 50µL standard or sample to each well.And then add 50µL prepared Detection Reagent A immediately.Shake and mix. Incubate 1 hour at 37°C;3. Aspirate and wash 3 times;4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;5. Aspirate and wash 5 times;6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;7. Add 50µL Stop Solution. Read at 450 nm immediately.
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Transforming Growth Factor Beta 2 (TGFb2) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Transforming Growth Factor Beta 2 (TGFb2) and unlabeled Transforming Growth Factor Beta 2 (TGFb2) (Standards or samples) with the pre-coated antibody specific to Transforming Growth Factor Beta 2 (TGFb2). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Transforming Growth Factor Beta 2 (TGFb2) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Transforming Growth Factor Beta 2 (TGFb2) in the sample.
ELISA / CLIA Experiment Service
Single-component Reagents of Assay KitLysis Buffer Specific for ELISA / CLIAQuality Control of ELISA / CLIAELISA Kit Customized ServiceDisease Model Customized ServiceSerums Customized ServiceTGFB1 Activation ReagentReal Time PCR Experimental Service
Catalog No. | Organism species: Mus musculus (Mouse) | Applications (RESEARCH USE ONLY!) |
RPA218Mu02 | Recombinant Transforming Growth Factor Beta 2 (TGFb2) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA218Mu01 | Recombinant Transforming Growth Factor Beta 2 (TGFb2) | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA218Mu02 | Polyclonal Antibody to Transforming Growth Factor Beta 2 (TGFb2) | WB; IHC; ICC; IP. |
PAA218Mu01 | Polyclonal Antibody to Transforming Growth Factor Beta 2 (TGFb2) | WB; IHC; ICC; IP. |
SEA218Mu | ELISA Kit for Transforming Growth Factor Beta 2 (TGFb2) | Enzyme-linked immunosorbent assay for Antigen Detection. |
MEA218Mu | Mini Samples ELISA Kit for Transforming Growth Factor Beta 2 (TGFb2) | Enzyme-linked immunosorbent assay for Antigen Detection. |
CEA218Mu | ELISA Kit for Transforming Growth Factor Beta 2 (TGFb2) | Enzyme-linked immunosorbent assay for Antigen Detection. |
在20mM Tris,150mM NaCl(pH8.0)中复溶至0.1-1.0 mg / mL的浓度。不要涡旋。
避免重复冷冻/解冻循环。在2-8°C下保存一个月。分装并在-80°C下存储12个月。
热稳定性由损失率描述。损失率通过加速热降解试验确定,即将蛋白质在37°C下孵育48h,没有观察到明显的降解和沉淀。在适当的存储条件下,有效期内的丢失率小于5%。