This assay has high sensitivity and excellent specificity for detection of Interleukin 17 (IL17).No significant cross-reactivity or interference between Interleukin 17 (IL17) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Interleukin 17 (IL17) and the recovery rates were calculated by comparing the measured value to the expected amount of Interleukin 17 (IL17) in samples.
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 79-98 | 95 |
EDTA plasma(n=5) | 85-97 | 93 |
heparin plasma(n=5) | 82-105 | 98 |
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 17 (IL17) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 17 (IL17) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10%>10%>Inter-Assay: CV<12%>12%>
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Interleukin 17 (IL17) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 94-102% | 78-95% | 89-98% | 91-98% |
EDTA plasma(n=5) | 89-102% | 78-95% | 85-102% | 79-89% |
heparin plasma(n=5) | 90-101% | 95-104% | 92-99% | 91-104% |
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
1. Prepare all reagents, samples and standards;2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;4. Aspirate and wash 3 times;5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;6. Aspirate and wash 5 times;7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;8. Read RLU value immediately.
The microplate provided in this kit has been pre-coated with an antibody specific to Interleukin 17 (IL17). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Interleukin 17 (IL17). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Interleukin 17 (IL17) level in the sample or standard.;
ELISA / CLIA Experiment Service
Single-component Reagents of Assay KitLysis Buffer Specific for ELISA / CLIAQuality Control of ELISA / CLIACLIA Kit Customized ServiceDisease Model Customized ServiceSerums Customized ServiceTGFB1 Activation ReagentReal Time PCR Experimental ServiceStreptavidin
Catalog No. | Organism species: Mus musculus (Mouse) | Applications (RESEARCH USE ONLY!) |
RPA063Mu02 | Recombinant Interleukin 17 (IL17) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA063Mu01 | Recombinant Interleukin 17 (IL17) | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA063Mu01 | Polyclonal Antibody to Interleukin 17 (IL17) | WB; IHC; ICC; IP. |
PAA063Mu02 | Polyclonal Antibody to Interleukin 17 (IL17) | WB; IHC; ICC; IP. |
MAA063Mu22 | Monoclonal Antibody to Interleukin 17 (IL17) | WB; IHC; ICC; IP. |
SEA063Mu | ELISA Kit for Interleukin 17 (IL17) | Enzyme-linked immunosorbent assay for Antigen Detection. |
MEA063Mu | Mini Samples ELISA Kit for Interleukin 17 (IL17) | Enzyme-linked immunosorbent assay for Antigen Detection. |
HEA063Mu | High Sensitive ELISA Kit for Interleukin 17 (IL17) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCA063Mu | CLIA Kit for Interleukin 17 (IL17) | Chemiluminescent immunoassay for Antigen Detection. |
在20mM Tris,150mM NaCl(pH8.0)中复溶至0.1-1.0 mg / mL的浓度。不要涡旋。
避免重复冷冻/解冻循环。在2-8°C下保存一个月。分装并在-80°C下存储12个月。
热稳定性由损失率描述。损失率通过加速热降解试验确定,即将蛋白质在37°C下孵育48h,没有观察到明显的降解和沉淀。在适当的存储条件下,有效期内的丢失率小于5%。