Figure 1. Cell apoptosis of A549 cells after stimulated by TNFa. <br/>TNFa, being an endogenous pyrogen, is able to induce fever, apoptotic cell death, inflammation and inhibit tumorigenesis. As reported, TNFa could inhibit the proliferation and induce apoptosis of A549 cells, and the concentration of IL-1β in cell supernatant will increase after stimulation. A549 cells were incubated in DMEM with TNFa (1ng/mL, 10ng/mL) for 2h, 4h, 8h, 24h, 48h, then cells were observed by inverted microscope and IL-1β in cell supernatant was detected by ELISA. Cell apoptosis of A549 after incubation of 48h was shown in Figure 1.<br/> (A)A549 cells cultured in DMEM, stimulated with 1ng/mL TNFa for 48h; <br/>(B)A549 cells cultured in DMEM, stimulated with 10ng/mL TNFa for 48h; <br/>(C)A549 cells cultured in DMEM for 48h.
Table 1. ELISA detection of IL-1β expression from A549 cells by TNFa.Effect of TNFa on the expression of IL-1β is shown in Table 1.
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Polyacrylamide Gel Electrophoresis (PAGE) Experiment Service
BCA Protein Quantification KitMolecular Mass Marker for ProteinMonoclonal Antibody Customized ServicePolyclonal Antibody Customized ServiceProtein Activity Test Experiment ServiceElectrophoretic Mobility Shift Assay (EMSA) Experiment ServiceBufferLentivirus Packaging Experiment ServiceAdenovirus Packaging Experiment ServiceReal Time PCR Experimental ServiceSpike RBD Protein (S-RBD)Protein GProtein A
Catalog No. | Organism species: Mus musculus (Mouse) | Applications (RESEARCH USE ONLY!) |
APA133Mu01 | Active Tumor Necrosis Factor Alpha (TNFa) | Cell culture; Activity Assays. |
APA133Mu61 | Active Tumor Necrosis Factor Alpha (TNFa) | Cell culture; Activity Assays. |
RPA133Mu02 | Recombinant Tumor Necrosis Factor Alpha (TNFa) | Positive Control; Immunogen; SDS-PAGE; WB. |
EPA133Mu61 | Eukaryotic Tumor Necrosis Factor Alpha (TNFa) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA133Mu01 | Recombinant Tumor Necrosis Factor Alpha (TNFa) | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA133Mu01 | Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa) | WB; IHC; ICC; IP. |
PAA133Mu02 | Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa) | WB; IHC; ICC; IP. |
PAA133Mu06 | Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa) | WB; IHC; ICC; IP. |
LAA133Mu71 | Biotin-Linked Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa) | WB; IHC; ICC. |
MAA133Mu21 | Monoclonal Antibody to Tumor Necrosis Factor Alpha (TNFa) | WB; IHC; ICC; IP. |
SEA133Mu | ELISA Kit for Tumor Necrosis Factor Alpha (TNFa) | Enzyme-linked immunosorbent assay for Antigen Detection. |
WEA133Mu | Wide-range ELISA Kit for Tumor Necrosis Factor Alpha (TNFa) | Enzyme-linked immunosorbent assay for Antigen Detection. |
MEA133Mu | Mini Samples ELISA Kit for Tumor Necrosis Factor Alpha (TNFa) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCA133Mu | CLIA Kit for Tumor Necrosis Factor Alpha (TNFa) | Chemiluminescent immunoassay for Antigen Detection. |
KSA133Mu01 | ELISA Kit DIY Materials for Tumor Necrosis Factor Alpha (TNFa) | Main materials for "Do It (ELISA Kit) Yourself". |
在20mM Tris,150mM NaCl(pH8.0)中复溶至0.1-1.0 mg / mL的浓度。不要涡旋。
避免重复冷冻/解冻循环。在2-8°C下保存一个月。分装并在-80°C下存储12个月。
热稳定性由损失率描述。损失率通过加速热降解试验确定,即将蛋白质在37°C下孵育48h,没有观察到明显的降解和沉淀。在适当的存储条件下,有效期内的丢失率小于5%。