Nerve growth factor (NGF) is a neurotrophic factor and neuropeptide primarily involved in the regulation of growth, maintenance, proliferation, and survival of certain target neurons. As reported, when the pheochromocytoma cell line PC12 is exposed to nerve growth factor (NGF), the cells respond over a period of a week by ceasing cell division and extending neurites (Greene and Tischler, 1976). The cells were grown in Ham"s F12K containing 5% fetal calf serum and 10% horse serum on polylysine or collagen coated plates. When cells reached log phase growth , fresh medium was added together with 10ng/mL of NGF, then cells were observed by inverted microscope everyday.Cell division ceasing and differentiation of PC12 cells after incubation with NGF (10ng/mL) for 6 days was shown in Figure1.Control group which received no NGF displayed no neurite outgrowth and cells multiply rapidly.
NGF is also involved in pathways besides those regulating the life cycle of neurons. Besides, Alpha-2-Macroglobulin (a2M) has been identified as an interactor of NGF, thus a binding ELISA assay was conducted to detect the interaction of recombinant human NGF and recombinant human a2M. Briefly, NGF were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μL were then transferred to a2M-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-NGF pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of NGF and a2M was shown in Figure 2, and this effect was in a dose dependent manner.Figure. The binding activity of NGF with a2M
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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Catalog No. | Organism species: Homo sapiens (Human) | Applications (RESEARCH USE ONLY!) |
RPA105Hu01 | Recombinant Nerve Growth Factor (NGF) | Positive Control; Immunogen; SDS-PAGE; WB. |
APA105Hu01 | Active Nerve Growth Factor (NGF) | Cell culture; Activity Assays. |
EPA105Hu61 | Eukaryotic Nerve Growth Factor (NGF) | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA105Hu01 | Polyclonal Antibody to Nerve Growth Factor (NGF) | WB; IHC; ICC; IP. |
PAA105Hu06 | Polyclonal Antibody to Nerve Growth Factor (NGF) | WB; IHC; ICC; IP. |
LAA105Hu71 | Biotin-Linked Polyclonal Antibody to Nerve Growth Factor (NGF) | WB; IHC; ICC. |
MAA105Hu21 | Monoclonal Antibody to Nerve Growth Factor (NGF) | WB; IHC; ICC; IP. |
MAA105Hu22 | Monoclonal Antibody to Nerve Growth Factor (NGF) | WB; IHC; ICC; IP. |
MAA105Hu23 | Monoclonal Antibody to Nerve Growth Factor (NGF) | WB; IHC; ICC; IP. |
SEA105Hu | ELISA Kit for Nerve Growth Factor (NGF) | Enzyme-linked immunosorbent assay for Antigen Detection. |
HEA105Hu | High Sensitive ELISA Kit for Nerve Growth Factor (NGF) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCA105Hu | CLIA Kit for Nerve Growth Factor (NGF) | Chemiluminescent immunoassay for Antigen Detection. |
KSA105Hu01 | ELISA Kit DIY Materials for Nerve Growth Factor (NGF) | Main materials for "Do It (ELISA Kit) Yourself". |
在20mM Tris,150mM NaCl(pH8.0)中复溶至0.1-1.0 mg / mL的浓度。不要涡旋。
避免重复冷冻/解冻循环。在2-8°C下保存一个月。分装并在-80°C下存储12个月。
热稳定性由损失率描述。损失率通过加速热降解试验确定,即将蛋白质在37°C下孵育48h,没有观察到明显的降解和沉淀。在适当的存储条件下,有效期内的丢失率小于5%。