Active Interleukin 10 (IL10)
CSIF; IL10A; TGIF; Cytokine Synthesis Inhibitory Factor
- Product No.APA056Hu61
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Caprine
- Ovine
- Equine
- Bovine
- Porcine
- Gallus
- Canine
- Others
- Multi-species
- Pan-species
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- TraitsFreeze-dried powder
- Purity> 97%
- Isoelectric Point7.6
- ApplicationsCell culture; Activity Assays.
- DownloadInstruction Manual
- UOM10µg50µg200µg1mg5mg
- FOBUS$ 335 For more details, please contact local distributors!US$ 837 For more details, please contact local distributors!US$ 1674 For more details, please contact local distributors!US$ 5022 For more details, please contact local distributors!US$ 12555 For more details, please contact local distributors!
Packages (Simulation)- Packages (Simulation)
- SDS-PAGE
- Figure. Western Blot; Sample:APA056Hu61; Antibody: PAA056Hu06
- ISO9001: 2008, ISO13485: 2003 Registered
ACTIVITY TEST of the Active Interleukin 10 (IL10)
Interleukin 10 (IL-10), also known as human cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine. IL-10 was initially reported to suppress cytokine secretion, antigen presentation and CD4T cell activation. Further investigation has shown that IL-10 predominantly inhibits lipopolysaccharide (LPS) mediated induction of the pro-inflammatory cytokines TNFα, IL-1β, IL-12, and IFNγ made by cells such as macrophages and Th1 T cells. Therefore, THP1 cells were cultured in 24 well plates at a concentration of 106 cells/ml and activated by LPS (1ug/mL) in the absence or presence of IL-10 (5ng/mL, 10ng/mL) for 24h and production of IL-1β was determined in the supernatants by cytokine specific ELISA.Activation of THP1 cells with LPS (1ug/mL) resulted in high levels of production of IL-1β and strong inhibitory effects of IL-10 (5ng/mL, 10ng/mL) were observed on the production of IL-1β as shown in Table 1.
USAGE of the Active Interleukin 10 (IL10)
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE of the Active Interleukin 10 (IL10)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active Interleukin 10 (IL10)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
Polyacrylamide Gel Electrophoresis (PAGE) Experiment Service
INCREMENT SERVICES
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Related products
| Catalog No. | Organism species: Homo sapiens (Human) | Applications (RESEARCH USE ONLY!) |
| APA056Hu01 | Active Interleukin 10 (IL10) | Cell culture; Activity Assays. |
| RPA056Hu01 | Recombinant Interleukin 10 (IL10) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA056Hu02 | Recombinant Interleukin 10 (IL10) | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA056Hu61 | Active Interleukin 10 (IL10) | Cell culture; Activity Assays. |
| EPA056Hu61 | Eukaryotic Interleukin 10 (IL10) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA056Hu02 | Polyclonal Antibody to Interleukin 10 (IL10) | WB; IHC; ICC; IP. |
| PAA056Hu01 | Polyclonal Antibody to Interleukin 10 (IL10) | WB; IHC; ICC; IP. |
| PAA056Hu06 | Polyclonal Antibody to Interleukin 10 (IL10) | WB; IHC; ICC; IP. |
| LAA056Hu71 | Biotin-Linked Polyclonal Antibody to Interleukin 10 (IL10) | WB; IHC; ICC. |
| MAA056Hu22 | Monoclonal Antibody to Interleukin 10 (IL10) | WB; IHC; ICC; IP. |
| MAA056Hu21 | Monoclonal Antibody to Interleukin 10 (IL10) | WB; IHC; ICC; IP. |
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| MEA056Hu | Mini Samples ELISA Kit for Interleukin 10 (IL10) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA056Hu | CLIA Kit for Interleukin 10 (IL10) | Chemiluminescent immunoassay for Antigen Detection. |
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Uscnk重组胃泌素（GT）
GAST；加油站; 胃泌素成分I；大胃泌素；胃泌素成分II; 胃泌素成分III
重组胃泌素（GT）的序列
重组胃泌素（GT）的用途
在20mM Tris，150mM NaCl（pH8.0）中复溶至0.1-1.0 mg / mL的浓度。不要涡旋。
重组胃泌素（GT）的存储
避免重复冷冻/解冻循环。在2-8°C下保存一个月。分装并在-80°C下存储12个月。
重组胃泌素（GT）的稳定性
热稳定性由损失率描述。损失率通过加速热降解试验确定，即将蛋白质在37°C下孵育48h，没有观察到明显的降解和沉淀。在适当的存储条件下，有效期内的丢失率小于5％。
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