Interleukin 4 (IL4) is a cytokine that induces differentiation of naive helper T cellsand monocyte. As reported, IL-4 would induce the differentiation of THP-1 cells into dendritic cells and macrophages in vitro. In house data was obtained by the following experiment :THP-1 cells were cultured in RPMI-1640 and stimulated with 2ng/mL IL-4, after 7 days of stimulation, cell bodies enlarged with extending pseudopodia, and vesicular bodies appeared within the cells, which showed a morphological characteristics of dendritic cells and macrophages.<br/>Cell differentiation of THP1 cells after incubation with IL4 (2ng/mL) for 7 days was shown in Figure 1.
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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Catalog No. | Organism species: Homo sapiens (Human) | Applications (RESEARCH USE ONLY!) |
RPA077Hu02 | Recombinant Interleukin 4 (IL4) | Positive Control; Immunogen; SDS-PAGE; WB. |
EPA077Hu61 | Eukaryotic Interleukin 4 (IL4) | Positive Control; Immunogen; SDS-PAGE; WB. |
APA077Hu61 | Active Interleukin 4 (IL4) | Cell culture; Activity Assays. |
APA077Hu01 | Active Interleukin 4 (IL4) | Cell culture; Activity Assays. |
RPA077Hu01 | Recombinant Interleukin 4 (IL4) | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA077Hu02 | Polyclonal Antibody to Interleukin 4 (IL4) | WB; IHC; ICC; IP. |
PAA077Hu06 | Polyclonal Antibody to Interleukin 4 (IL4) | WB; IHC; ICC; IP. |
PAA077Hu01 | Polyclonal Antibody to Interleukin 4 (IL4) | WB; IHC; ICC; IP. |
MAA077Hu22 | Monoclonal Antibody to Interleukin 4 (IL4) | WB; IHC; ICC; IP. |
SEA077Hu | ELISA Kit for Interleukin 4 (IL4) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCA077Hu | CLIA Kit for Interleukin 4 (IL4) | Chemiluminescent immunoassay for Antigen Detection. |
KSA077Hu01 | ELISA Kit DIY Materials for Interleukin 4 (IL4) | Main materials for "Do It (ELISA Kit) Yourself". |
在20mM Tris,150mM NaCl(pH8.0)中复溶至0.1-1.0 mg / mL的浓度。不要涡旋。
避免重复冷冻/解冻循环。在2-8°C下保存一个月。分装并在-80°C下存储12个月。
热稳定性由损失率描述。损失率通过加速热降解试验确定,即将蛋白质在37°C下孵育48h,没有观察到明显的降解和沉淀。在适当的存储条件下,有效期内的丢失率小于5%。