Measured in a cell proliferation assay using mouse BMDC (bone marrow derived dendritic cells). The ED50 (median effective dose) for this effect is less than 0.25 ng/mL.In-house data of APA045Mu01 used in cellular experiment:Six-eight weeks old Balb/c mice were used for BMDC. At first, mouse femur and tibia were taken out, and then bone marrow was washed out with serum-free RPMI 1640 medium, followed by centrifugation at 1200RPM for 5min (4oC). ACK buffer was added to get rid of red blood cells, and then, centrifuged at 1200RPM for 5min (4oC). Cell pellets were collected and re-suspended, the cells were cultured in DMEM medium supplemented with 10% FBS, or DMEM medium supplemented with 10% FBS and GMCSF (APA045Mu01) at 37oC with 5% CO2 in thermostatic incubator. Three days later, cells were observed by microscope, and the result is shown in Figure 1.
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Polyacrylamide Gel Electrophoresis (PAGE) Experiment Service
BCA Protein Quantification KitMolecular Mass Marker for ProteinMonoclonal Antibody Customized ServicePolyclonal Antibody Customized ServiceProtein Activity Test Experiment ServiceElectrophoretic Mobility Shift Assay (EMSA) Experiment ServiceBufferLentivirus Packaging Experiment ServiceAdenovirus Packaging Experiment ServiceReal Time PCR Experimental ServiceSpike RBD Protein (S-RBD)Protein GProtein A
Catalog No. | Organism species: Mus musculus (Mouse) | Applications (RESEARCH USE ONLY!) |
RPA045Mu02 | Recombinant Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF) | Positive Control; Immunogen; SDS-PAGE; WB. |
APA045Mu01 | Active Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF) | Cell culture; Activity Assays. |
RPA045Mu01 | Recombinant Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF) | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA045Mu01 | Polyclonal Antibody to Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF) | WB; IHC; ICC; IP. |
PAA045Mu02 | Polyclonal Antibody to Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF) | WB; IHC; ICC; IP. |
SEA045Mu | ELISA Kit for Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF) | Enzyme-linked immunosorbent assay for Antigen Detection. |
MEA045Mu | Mini Samples ELISA Kit for Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCA045Mu | CLIA Kit for Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF) | Chemiluminescent immunoassay for Antigen Detection. |
KSA045Mu01 | ELISA Kit DIY Materials for Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF) | Main materials for "Do It (ELISA Kit) Yourself". |
在20mM Tris,150mM NaCl(pH8.0)中复溶至0.1-1.0 mg / mL的浓度。不要涡旋。
避免重复冷冻/解冻循环。在2-8°C下保存一个月。分装并在-80°C下存储12个月。
热稳定性由损失率描述。损失率通过加速热降解试验确定,即将蛋白质在37°C下孵育48h,没有观察到明显的降解和沉淀。在适当的存储条件下,有效期内的丢失率小于5%。