MIP-1a (macrophage inflammatory protein 1-alpha) also known as Chemokine (C-C motief) ligand 3 (CCL3), is a cytokine belonging to the CC chemokine family that is involved in the recruitment and activation of macrophages, monocytes and neutrophils. In this case, chemotaxis assay used 24-well microchemotaxis system was undertaken to evaluate the chemotactic effect of MIP-1a on the human monocytic cell line THP1. Briefly, THP1 cells were seeded into the upper chambers (100µl cell suspension, 106 cells/ml in RPMI 1640 with 0.5% FBS) and MIP-1a (100ng/mL, diluted in serum free RPMI 1640 ) was added in lower chamber with a polycarbonate filter (8µm pore size) used to separate the two compartments. After incubation at 37oC with 5% CO2 for 5h, the filter was removed, then cells in low chamber were observed by inverted microscope at low magnification (×40) and the number of migrated cells were counted at high magnification (×400) randomly (five fields for each filter).By counting migrated cells in low chamber at high magnification (×400) randomly, it was shown that a mean of 41.2 THP1 cells/field migrated towards serum free RPMI 1640 medium with 100ng/mL MIP-1a, while only 3.6 THP1 cells/field migrated towards serum free RPMI 1640 medium. And the migrated THP1 cells in low chamber at low magnification (×40) was shown in Figure 1.
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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Catalog No. | Organism species: Homo sapiens (Human) | Applications (RESEARCH USE ONLY!) |
RPA092Hu01 | Recombinant Macrophage Inflammatory Protein 1 Alpha (MIP1a) | Positive Control; Immunogen; SDS-PAGE; WB. |
APA092Hu01 | Active Macrophage Inflammatory Protein 1 Alpha (MIP1a) | Cell culture; Activity Assays. |
APA092Hu61 | Active Macrophage Inflammatory Protein 1 Alpha (MIP1a) | Cell culture; Activity Assays. |
EPA092Hu61 | Eukaryotic Macrophage Inflammatory Protein 1 Alpha (MIP1a) | Positive Control; Immunogen; SDS-PAGE; WB. |
EPA092Hu51 | Eukaryotic Macrophage Inflammatory Protein 1 Alpha (MIP1a) | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA092Hu07 | Polyclonal Antibody to Macrophage Inflammatory Protein 1 Alpha (MIP1a) | WB; IHC; ICC; IP. |
PAA092Hu01 | Polyclonal Antibody to Macrophage Inflammatory Protein 1 Alpha (MIP1a) | WB; IHC; ICC; IP. |
PAA092Hu06 | Polyclonal Antibody to Macrophage Inflammatory Protein 1 Alpha (MIP1a) | WB; IHC; ICC; IP. |
MAA092Hu22 | Monoclonal Antibody to Macrophage Inflammatory Protein 1 Alpha (MIP1a) | WB; IHC; ICC; IP. |
SEA092Hu | ELISA Kit for Macrophage Inflammatory Protein 1 Alpha (MIP1a) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCA092Hu | CLIA Kit for Macrophage Inflammatory Protein 1 Alpha (MIP1a) | Chemiluminescent immunoassay for Antigen Detection. |
KSA092Hu01 | ELISA Kit DIY Materials for Macrophage Inflammatory Protein 1 Alpha (MIP1a) | Main materials for "Do It (ELISA Kit) Yourself". |
PGA092Hu01 | Primer Pair for Macrophage Inflammatory Protein 1 Alpha (MIP1a) | PCR |
在20mM Tris,150mM NaCl(pH8.0)中复溶至0.1-1.0 mg / mL的浓度。不要涡旋。
避免重复冷冻/解冻循环。在2-8°C下保存一个月。分装并在-80°C下存储12个月。
热稳定性由损失率描述。损失率通过加速热降解试验确定,即将蛋白质在37°C下孵育48h,没有观察到明显的降解和沉淀。在适当的存储条件下,有效期内的丢失率小于5%。