IL1α (Interleukin-1 alpha) is a member of the interleukin 1 cytokine family. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces cell apoptosis. It is reported that exposure of MCF-7 cells to certain concentration of IL1α results in inhibition of cell growth. Thus, an cell proliferation assay of MCF-7 was conducted with the addition of IL1α. MCF-7 cells were seeded overnight at a density of 5,000 cells/well, and then treated with or without various concentrations of IL1α for 72h, then cells were observed by inverted microscope and cell viability was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10µL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours in at 37°C.<br/>Inhibition of MCF-7 cell proliferation after incubation with IL1α for 72h observed by inverted microscope was shown in Figure 1.
Cell viability was assessed by CCK-8 (Cell Counting Kit-8) assay after incubation with various concentrations of IL1α for 72h. The mean OD value of MCF-7 assessed by CCK-8 was shown in Figure 2. It was obvious that IL1α significantly decreased cell viability of MCF-7 cells.
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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Catalog No. | Organism species: Homo sapiens (Human) | Applications (RESEARCH USE ONLY!) |
RPA071Hu02 | Recombinant Interleukin 1 Alpha (IL1a) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA071Hu03 | Recombinant Interleukin 1 Alpha (IL1a) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA071Hu01 | Recombinant Interleukin 1 Alpha (IL1a) | Positive Control; Immunogen; SDS-PAGE; WB. |
APA071Hu02 | Active Interleukin 1 Alpha (IL1a) | Cell culture; Activity Assays. |
APA071Hu01 | Active Interleukin 1 Alpha (IL1a) | Cell culture; Activity Assays. |
PAA071Hu02 | Polyclonal Antibody to Interleukin 1 Alpha (IL1a) | WB; IHC; ICC; IP. |
PAA071Hu01 | Polyclonal Antibody to Interleukin 1 Alpha (IL1a) | WB; IHC; ICC; IP. |
LAA071Hu71 | Biotin-Linked Polyclonal Antibody to Interleukin 1 Alpha (IL1a) | WB; IHC; ICC. |
LAA071Hu81 | FITC-Linked Polyclonal Antibody to Interleukin 1 Alpha (IL1a) | WB; IHC; ICC; IF. |
MAA071Hu22 | Monoclonal Antibody to Interleukin 1 Alpha (IL1a) | WB; IHC; ICC; IP. |
MAA071Hu21 | Monoclonal Antibody to Interleukin 1 Alpha (IL1a) | WB; IHC; ICC; IP. |
LAA071Hu72 | Biotin-Linked Monoclonal Antibody to Interleukin 1 Alpha (IL1a) | WB; IHC; ICC. |
SEA071Hu | ELISA Kit for Interleukin 1 Alpha (IL1a) | Enzyme-linked immunosorbent assay for Antigen Detection. |
PSA071Hu01 | Antibody Pair for Interleukin 1 Alpha (IL1a) | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
KSA071Hu01 | ELISA Kit DIY Materials for Interleukin 1 Alpha (IL1a) | Main materials for "Do It (ELISA Kit) Yourself". |
IGA071Hu21 | Mouse Knockin Human Interleukin 1 Alpha (IL1a) | Animal Experiments; Positive Control. |
在20mM Tris,150mM NaCl(pH8.0)中复溶至0.1-1.0 mg / mL的浓度。不要涡旋。
避免重复冷冻/解冻循环。在2-8°C下保存一个月。分装并在-80°C下存储12个月。
热稳定性由损失率描述。损失率通过加速热降解试验确定,即将蛋白质在37°C下孵育48h,没有观察到明显的降解和沉淀。在适当的存储条件下,有效期内的丢失率小于5%。