Myostatin (MSTN) also known as growth differentiation factor 8 (GDF-8) is a member of the TGF beta protein family. Myostatin is a secreted growth differentiation factor that produced and released by myocytes. This protein negatively regulates skeletal muscle cell proliferation and differentiation. Besides, Bone Morphogenetic Protein 1 (BMP1) has been identified as an interactor of MSTN, thus a binding ELISA assay was conducted to detect the interaction of recombinant human MSTN and recombinant human BMP1. Briefly, MSTN were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to BMP1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-MSTN pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of MSTN and BMP1 was shown in Figure 1, and this effect was in a dose dependent manner.Figure. The binding activity of MSTN with BMP1.
Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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Catalog No. | Organism species: Homo sapiens (Human) | Applications (RESEARCH USE ONLY!) |
APB653Hu01 | Active Myostatin (MSTN) | Cell culture; Activity Assays. |
EPB653Hu61 | Eukaryotic Myostatin (MSTN) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPB653Hu01 | Recombinant Myostatin (MSTN) | Positive Control; Immunogen; SDS-PAGE; WB. |
APB653Hu61 | Active Myostatin (MSTN) | Cell culture; Activity Assays. |
CPB653Hu21 | OVA Conjugated Myostatin (MSTN) | Immunogen; SDS-PAGE; WB. |
PAB653Hu08 | Polyclonal Antibody to Myostatin (MSTN) | WB; IHC; ICC; IP. |
PAB653Hu06 | Polyclonal Antibody to Myostatin (MSTN) | WB; IHC; ICC; IP. |
PAB653Hu01 | Polyclonal Antibody to Myostatin (MSTN) | WB; IHC; ICC; IP. |
LAB653Hu71 | Biotin-Linked Polyclonal Antibody to Myostatin (MSTN) | WB; IHC; ICC. |
MAB653Hu22 | Monoclonal Antibody to Myostatin (MSTN) | WB; IHC; ICC; IP. |
MAB653Hu21 | Monoclonal Antibody to Myostatin (MSTN) | WB; IHC; ICC; IP. |
SEB653Hu | ELISA Kit for Myostatin (MSTN) | Enzyme-linked immunosorbent assay for Antigen Detection. |
CEB653Hu | ELISA Kit for Myostatin (MSTN) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCB653Hu | CLIA Kit for Myostatin (MSTN) | Chemiluminescent immunoassay for Antigen Detection. |
KSB653Hu11 | ELISA Kit DIY Materials for Myostatin (MSTN) | Main materials for "Do It (ELISA Kit) Yourself". |
在20mM Tris,150mM NaCl(pH8.0)中复溶至0.1-1.0 mg / mL的浓度。不要涡旋。
避免重复冷冻/解冻循环。在2-8°C下保存一个月。分装并在-80°C下存储12个月。
热稳定性由损失率描述。损失率通过加速热降解试验确定,即将蛋白质在37°C下孵育48h,没有观察到明显的降解和沉淀。在适当的存储条件下,有效期内的丢失率小于5%。