The antibody isa rabbit polyclonal antibody raised against EGF. It has been selected for its ability to recognize EGF in immunohistochemical staining and western blotting.
Western blotting: 0.5-2µg/mLImmunohistochemistry: 5-20µg/mLImmunocytochemistry: 5-20µg/mLOptimal working dilutions must be determined by end user.
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Antibody Labeling Customized ServiceProtein A/G Purification ColumnStaining Solution for Cells and TissuePositive Controlfor AntibodyTissue/Sections Customized ServicePhosphorylated Antibody Customized ServiceWestern Blot (WB) Experiment ServiceImmunohistochemistry (IHC) Experiment ServiceImmunocytochemistry (ICC) Experiment ServiceFlow Cytometry (FCM) Experiment ServiceImmunoprecipitation (IP) Experiment ServiceImmunofluorescence (IF) Experiment ServiceBufferDAB Chromogen KitSABC KitLong-arm Biotin Labeling KitReal Time PCR Experimental Service
Catalog No. | Organism species: Mus musculus (Mouse) | Applications (RESEARCH USE ONLY!) |
RPA560Mu02 | Recombinant Epidermal Growth Factor (EGF) | Positive Control; Immunogen; SDS-PAGE; WB. |
APA560Mu01 | Active Epidermal Growth Factor (EGF) | Cell culture; Activity Assays. |
RPA560Mu01 | Recombinant Epidermal Growth Factor (EGF) | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA560Mu02 | Polyclonal Antibody to Epidermal Growth Factor (EGF) | WB; IHC; ICC; IP. |
PAA560Mu01 | Polyclonal Antibody to Epidermal Growth Factor (EGF) | WB; IHC; ICC; IP. |
SEA560Mu | ELISA Kit for Epidermal Growth Factor (EGF) | Enzyme-linked immunosorbent assay for Antigen Detection. |
MEA560Mu | Mini Samples ELISA Kit for Epidermal Growth Factor (EGF) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCA560Mu | CLIA Kit for Epidermal Growth Factor (EGF) | Chemiluminescent immunoassay for Antigen Detection. |
在20mM Tris,150mM NaCl(pH8.0)中复溶至0.1-1.0 mg / mL的浓度。不要涡旋。
避免重复冷冻/解冻循环。在2-8°C下保存一个月。分装并在-80°C下存储12个月。
热稳定性由损失率描述。损失率通过加速热降解试验确定,即将蛋白质在37°C下孵育48h,没有观察到明显的降解和沉淀。在适当的存储条件下,有效期内的丢失率小于5%。