The antibody isa mouse monoclonal antibody raised against BDNF. It has been selected for its ability to recognize BDNF in immunohistochemical staining and western blotting.
Western blotting: 0.2-2µg/mL;1:500-5000 Immunohistochemistry: 5-20µg/mL;1:50-200 Immunocytochemistry: 5-20µg/mL;1:50-200 Optimal working dilutions must be determined by end user.
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Antibody Labeling Customized ServiceProtein A/G Purification ColumnStaining Solution for Cells and TissuePositive Controlfor AntibodyTissue/Sections Customized ServicePhosphorylated Antibody Customized ServiceWestern Blot (WB) Experiment ServiceImmunohistochemistry (IHC) Experiment ServiceImmunocytochemistry (ICC) Experiment ServiceFlow Cytometry (FCM) Experiment ServiceImmunoprecipitation (IP) Experiment ServiceImmunofluorescence (IF) Experiment ServiceBufferDAB Chromogen KitSABC KitLong-arm Biotin Labeling KitReal Time PCR Experimental Service
Catalog No. | Organism species: Homo sapiens (Human) | Applications (RESEARCH USE ONLY!) |
RPA011Hu01 | Recombinant Brain Derived Neurotrophic Factor (BDNF) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA011Hu02 | Recombinant Brain Derived Neurotrophic Factor (BDNF) | Positive Control; Immunogen; SDS-PAGE; WB. |
APA011Hu61 | Active Brain Derived Neurotrophic Factor (BDNF) | Cell culture; Activity Assays. |
EPA011Hu61 | Eukaryotic Brain Derived Neurotrophic Factor (BDNF) | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA011Hu01 | Polyclonal Antibody to Brain Derived Neurotrophic Factor (BDNF) | WB; IHC; ICC; IP. |
PAA011Hu06 | Polyclonal Antibody to Brain Derived Neurotrophic Factor (BDNF) | WB; IHC; ICC; IP. |
LAA011Hu71 | Biotin-Linked Polyclonal Antibody to Brain Derived Neurotrophic Factor (BDNF) | WB; IHC; ICC. |
LAA011Hu81 | FITC-Linked Polyclonal Antibody to Brain Derived Neurotrophic Factor (BDNF) | WB; IHC; ICC; IF. |
MAA011Hu22 | Monoclonal Antibody to Brain Derived Neurotrophic Factor (BDNF) | WB; IHC; ICC; IP. |
SEA011Hu | ELISA Kit for Brain Derived Neurotrophic Factor (BDNF) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCA011Hu | CLIA Kit for Brain Derived Neurotrophic Factor (BDNF) | Chemiluminescent immunoassay for Antigen Detection. |
KSA011Hu01 | ELISA Kit DIY Materials for Brain Derived Neurotrophic Factor (BDNF) | Main materials for "Do It (ELISA Kit) Yourself". |
在20mM Tris,150mM NaCl(pH8.0)中复溶至0.1-1.0 mg / mL的浓度。不要涡旋。
避免重复冷冻/解冻循环。在2-8°C下保存一个月。分装并在-80°C下存储12个月。
热稳定性由损失率描述。损失率通过加速热降解试验确定,即将蛋白质在37°C下孵育48h,没有观察到明显的降解和沉淀。在适当的存储条件下,有效期内的丢失率小于5%。