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主营:分子类,蛋白类,抗体类,生化类试剂
℡ 4000-520-616
℡ 4000-520-616
Uscnk/ELISA Kit for Fibroblast Growth Factor 2, Basic (FGF2)/96T*100/CEA551Hu
产品编号:CEA551Hu
市  场 价:¥655200.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$32760.00
品      牌: Uscnk
公      司:Uscnk
公司分类:
Uscnk/ELISA Kit for Fibroblast Growth Factor 2, Basic (FGF2)/96T*100/CEA551Hu
商品介绍

ELISA Kit for Fibroblast Growth Factor 2, Basic (FGF2)

B-FGF; BFGF; FGFB; HBGH-2; Basic Fibroblast Growth Factor; Heparin-binding growth factor 2

  • Cytokine
  • Tumor immunity
  • Infection immunity
  • Featured-Product
  • Product No.CEA551Hu
  • Organism SpeciesHomo sapiens (Human) Same name, Different species.
    • All
    • Human
    • Mouse
    • Rat
    • Cavia
    • Rabbit
    • Simian
    • Caprine
    • Ovine
    • Equine
    • Bovine
    • Porcine
    • Gallus
    • Canine
    • Others
    • Multi-species
    • Pan-species
  • Test MethodCompetitive Inhibition
  • Assay Length2h
  • Detection Range12.35-1,000pg/mL
  • SensitivityThe minimum detectable dose of this kit is typically less than 5.03pg/mL.
  • Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • DownloadInstruction Manual
  • UOM48T96T96T*596T*1096T*100
  • FOBUS$ 328 For more details, please contact local distributors!US$ 468 For more details, please contact local distributors!US$ 2106 For more details, please contact local distributors!US$ 3978 For more details, please contact local distributors!US$ 32760 For more details, please contact local distributors!
  • ELISA Kit for Fibroblast Growth Factor 2, Basic (FGF2)Packages (Simulation)
  • ELISA Kit for Fibroblast Growth Factor 2, Basic (FGF2)Packages (Simulation)
  • ELISA Kit for Fibroblast Growth Factor 2, Basic (FGF2)Results demonstration
  • CEA551Hu.jpgTypical Standard Curve
  • CertificateISO9001: 2008, ISO13485: 2003 Registered

Specificity of the ELISA Kit for Fibroblast Growth Factor 2, Basic (FGF2)

This assay has high sensitivity and excellent specificity for detection of Fibroblast Growth Factor 2, Basic (FGF2).No significant cross-reactivity or interference between Fibroblast Growth Factor 2, Basic (FGF2) and analogues was observed.

Precision of the ELISA Kit for Fibroblast Growth Factor 2, Basic (FGF2)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibroblast Growth Factor 2, Basic (FGF2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibroblast Growth Factor 2, Basic (FGF2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10%>Inter-Assay: CV<12%>

Stability of the ELISA Kit for Fibroblast Growth Factor 2, Basic (FGF2)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the ELISA Kit for Fibroblast Growth Factor 2, Basic (FGF2)

1. Prepare all reagents, samples and standards;2. Add 50µL standard or sample to each well.And then add 50µL prepared Detection Reagent A immediately.Shake and mix. Incubate 1 hour at 37°C;3. Aspirate and wash 3 times;4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;5. Aspirate and wash 5 times;6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;7. Add 50µL Stop Solution. Read at 450 nm immediately.

Test principle of the ELISA Kit for Fibroblast Growth Factor 2, Basic (FGF2)

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Fibroblast Growth Factor 2, Basic (FGF2) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Fibroblast Growth Factor 2, Basic (FGF2) and unlabeled Fibroblast Growth Factor 2, Basic (FGF2) (Standards or samples) with the pre-coated antibody specific to Fibroblast Growth Factor 2, Basic (FGF2). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Fibroblast Growth Factor 2, Basic (FGF2) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Fibroblast Growth Factor 2, Basic (FGF2) in the sample.

GIVEAWAYS

ELISA / CLIA Experiment Service

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Related products

Catalog No.Organism species: Homo sapiens (Human) Applications (RESEARCH USE ONLY!)
RPA551Hu01Recombinant Fibroblast Growth Factor 2, Basic (FGF2)Positive Control; Immunogen; SDS-PAGE; WB.
APA551Hu01Active Fibroblast Growth Factor 2, Basic (FGF2)Cell culture; Activity Assays.
CPA551Hu21OVA Conjugated Fibroblast Growth Factor 2, Basic (FGF2)Immunogen; SDS-PAGE; WB.
PAA551Hu01Polyclonal Antibody to Fibroblast Growth Factor 2, Basic (FGF2)WB; IHC; ICC; IP.
LAA551Hu71Biotin-Linked Polyclonal Antibody to Fibroblast Growth Factor 2, Basic (FGF2)WB; IHC; ICC.
LAA551Hu81FITC-Linked Polyclonal Antibody to Fibroblast Growth Factor 2, Basic (FGF2)WB; IHC; ICC; IF.
MAA551Hu22Monoclonal Antibody to Fibroblast Growth Factor 2, Basic (FGF2)WB; IHC; ICC; IP.
SEA551HuELISA Kit for Fibroblast Growth Factor 2, Basic (FGF2)Enzyme-linked immunosorbent assay for Antigen Detection.
MEA551HuMini Samples ELISA Kit for Fibroblast Growth Factor 2, Basic (FGF2)Enzyme-linked immunosorbent assay for Antigen Detection.
CEA551HuELISA Kit for Fibroblast Growth Factor 2, Basic (FGF2)Enzyme-linked immunosorbent assay for Antigen Detection.
KSA551Hu11ELISA Kit DIY Materials for Fibroblast Growth Factor 2, Basic (FGF2)Main materials for "Do It (ELISA Kit) Yourself".
GUESTBOOK
品牌介绍

Uscnk重组胃泌素(GT)

GAST;加油站; 胃泌素成分I;大胃泌素;胃泌素成分II; 胃泌素成分III

  • 产品编号

  • RPB224Mu01

  • 生物物种小家鼠(Mouse musculus)(鼠)同名,不同种。

  • 资源原核表达

  • 主办大肠杆菌

  • 内毒素水平每1µg <1.0EU(通过LAL方法确定)

  • 亚细胞定位秘密的

  • 预测分子量42.6kDa的

  • 准确的分子质量43kDa(差异分析请参见手册)

  • 残基和标签Net His和GST标签的Met1〜Gln101

  • 缓冲液配方20mM Tris,150mM NaCl,pH8.0,包含1mM EDTA,1mM DTT,0.01%SKL,5%海藻糖和Proclin300。

  • 特质冻干粉

  • 纯度> 90%

  • 等电点6.3

  • 应用领域积极控制;免疫原 SDS-PAGE;WB。
    如果需要蛋白质的生物活性,请检查活性蛋白质。

  • 下载使用说明书

  • 单位10微克为50μg 200μg的 1mg的 5毫克

  • 离岸价US $ 480



重组胃泌素(GT)的序列




重组胃泌素(GT)的用途

在20mM Tris,150mM NaCl(pH8.0)中复溶至0.1-1.0 mg / mL的浓度。不要涡旋。

重组胃泌素(GT)的存储

避免重复冷冻/解冻循环。在2-8°C下保存一个月。分装并在-80°C下存储12个月。

重组胃泌素(GT)的稳定性

热稳定性由损失率描述。损失率通过加速热降解试验确定,即将蛋白质在37°C下孵育48h,没有观察到明显的降解和沉淀。在适当的存储条件下,有效期内的丢失率小于5%。

礼物

聚丙烯酰胺凝胶电泳(PAGE)实验服务


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