ELISA Kit for Fibroblast Growth Factor 23 (FGF23)
ADHR; HYPF; HPDR2; PHPTC; Phosphatonin; Tumor-derived hypophosphatemia-inducing factor
- Product No.CEA746Ra
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Caprine
- Ovine
- Equine
- Bovine
- Porcine
- Gallus
- Canine
- Others
- Multi-species
- Pan-species
- Test MethodCompetitive Inhibition
- Assay Length2h
- Detection Range2.47-200pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.92pg/mL.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- DownloadInstruction Manual
- UOM48T96T96T*596T*1096T*100
- FOBUS$ 479 For more details, please contact local distributors!US$ 684 For more details, please contact local distributors!US$ 3078 For more details, please contact local distributors!US$ 5814 For more details, please contact local distributors!US$ 47880 For more details, please contact local distributors!
Packages (Simulation)- Packages (Simulation)
- Results demonstration
- Typical Standard Curve
- ISO9001: 2008, ISO13485: 2003 Registered
Specificity of the ELISA Kit for Fibroblast Growth Factor 23 (FGF23)
This assay has high sensitivity and excellent specificity for detection of Fibroblast Growth Factor 23 (FGF23).No significant cross-reactivity or interference between Fibroblast Growth Factor 23 (FGF23) and analogues was observed.
Recovery of the ELISA Kit for Fibroblast Growth Factor 23 (FGF23)
Matrices listed below were spiked with certain level of recombinant Fibroblast Growth Factor 23 (FGF23) and the recovery rates were calculated by comparing the measured value to the expected amount of Fibroblast Growth Factor 23 (FGF23) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 97-105 | 102 |
| EDTA plasma(n=5) | 80-99 | 91 |
| heparin plasma(n=5) | 97-105 | 101 |
Precision of the ELISA Kit for Fibroblast Growth Factor 23 (FGF23)
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibroblast Growth Factor 23 (FGF23) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibroblast Growth Factor 23 (FGF23) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10%>Inter-Assay: CV<12%>
Linearity of the ELISA Kit for Fibroblast Growth Factor 23 (FGF23)
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Fibroblast Growth Factor 23 (FGF23) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 95-103% | 87-96% | 93-105% | 80-92% |
| EDTA plasma(n=5) | 98-105% | 93-101% | 80-89% | 80-104% |
| heparin plasma(n=5) | 88-96% | 80-105% | 96-105% | 85-104% |
Stability of the ELISA Kit for Fibroblast Growth Factor 23 (FGF23)
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary of the ELISA Kit for Fibroblast Growth Factor 23 (FGF23)
1. Prepare all reagents, samples and standards;2. Add 50µL standard or sample to each well.And then add 50µL prepared Detection Reagent A immediately.Shake and mix. Incubate 1 hour at 37°C;3. Aspirate and wash 3 times;4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;5. Aspirate and wash 5 times;6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;7. Add 50µL Stop Solution. Read at 450 nm immediately.
Test principle of the ELISA Kit for Fibroblast Growth Factor 23 (FGF23)
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Fibroblast Growth Factor 23 (FGF23) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Fibroblast Growth Factor 23 (FGF23) and unlabeled Fibroblast Growth Factor 23 (FGF23) (Standards or samples) with the pre-coated antibody specific to Fibroblast Growth Factor 23 (FGF23). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Fibroblast Growth Factor 23 (FGF23) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Fibroblast Growth Factor 23 (FGF23) in the sample.
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Related products
| Catalog No. | Organism species: Rattus norvegicus (Rat) | Applications (RESEARCH USE ONLY!) |
| RPA746Ra01 | Recombinant Fibroblast Growth Factor 23 (FGF23) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA746Ra01 | Polyclonal Antibody to Fibroblast Growth Factor 23 (FGF23) | WB; IHC; ICC; IP. |
| MAA746Ra21 | Monoclonal Antibody to Fibroblast Growth Factor 23 (FGF23) | WB; IHC; ICC; IP. |
| SEA746Ra | ELISA Kit for Fibroblast Growth Factor 23 (FGF23) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CEA746Ra | ELISA Kit for Fibroblast Growth Factor 23 (FGF23) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| KSA746Ra11 | ELISA Kit DIY Materials for Fibroblast Growth Factor 23 (FGF23) | Main materials for "Do It (ELISA Kit) Yourself". |
Uscnk重组胃泌素（GT）
GAST；加油站; 胃泌素成分I；大胃泌素；胃泌素成分II; 胃泌素成分III
重组胃泌素（GT）的序列
重组胃泌素（GT）的用途
在20mM Tris，150mM NaCl（pH8.0）中复溶至0.1-1.0 mg / mL的浓度。不要涡旋。
重组胃泌素（GT）的存储
避免重复冷冻/解冻循环。在2-8°C下保存一个月。分装并在-80°C下存储12个月。
重组胃泌素（GT）的稳定性
热稳定性由损失率描述。损失率通过加速热降解试验确定，即将蛋白质在37°C下孵育48h，没有观察到明显的降解和沉淀。在适当的存储条件下，有效期内的丢失率小于5％。
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