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主营:分子类,蛋白类,抗体类,生化类试剂
℡ 4000-520-616
℡ 4000-520-616
Uscnk/ELISA Kit for Anti-Tumor Necrosis Factor Alpha Antibody (Anti-TNFa)/96T*100/AEA133Hu
产品编号:AEA133Hu
市  场 价:¥1260000.00
场      地:美国(厂家直采)
产品分类: 抗体类>一抗>单克隆抗体>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$63000.00
品      牌: Uscnk
公      司:Uscnk
公司分类:
Uscnk/ELISA Kit for Anti-Tumor Necrosis Factor Alpha Antibody (Anti-TNFa)/96T*100/AEA133Hu
商品介绍

ELISA Kit for Anti-Tumor Necrosis Factor Alpha Antibody (Anti-TNFa)

DIF; TNF-A; TNFSF2; Cachectin; Tumor Necrosis Factor Ligand Superfamily Member 2

  • Cytokine
  • Tumor immunity
  • Infection immunity
  • Product No.AEA133Hu
  • Organism SpeciesHomo sapiens (Human) Same name, Different species.
    • All
    • Human
    • Mouse
    • Rat
    • Cavia
    • Rabbit
    • Simian
    • Caprine
    • Ovine
    • Equine
    • Bovine
    • Porcine
    • Gallus
    • Canine
    • Others
    • Multi-species
    • Pan-species
  • Test MethodIndirect ELISA
  • Assay Length2h, 30min
  • Detection Range3.12-200ng/mL
  • SensitivityThe minimum detectable dose of this kit is typically less than 1.13ng/mL.
  • Sample TypeSerum, plasma and other biological fluids
  • DownloadInstruction Manual
  • UOM48T96T96T*596T*1096T*100
  • FOBUS$ 630 For more details, please contact local distributors!US$ 900 For more details, please contact local distributors!US$ 4050 For more details, please contact local distributors!US$ 7650 For more details, please contact local distributors!US$ 63000 For more details, please contact local distributors!
  • ELISA Kit for Anti-Tumor Necrosis Factor Alpha Antibody (Anti-TNFa)Packages (Simulation)
  • ELISA Kit for Anti-Tumor Necrosis Factor Alpha Antibody (Anti-TNFa)Packages (Simulation)
  • ELISA Kit for Anti-Tumor Necrosis Factor Alpha Antibody (Anti-TNFa)Results demonstration
  • AEA133Hu.jpgTypical Standard Curve
  • CertificateISO9001: 2008, ISO13485: 2003 Registered

Specificity of the ELISA Kit for Anti-Tumor Necrosis Factor Alpha Antibody (Anti-TNFa)

This assay has high sensitivity and excellent specificity for detection of Anti-Tumor Necrosis Factor Alpha Antibody (Anti-TNFa).No significant cross-reactivity or interference between Anti-Tumor Necrosis Factor Alpha Antibody (Anti-TNFa) and analogues was observed.

Recovery of the ELISA Kit for Anti-Tumor Necrosis Factor Alpha Antibody (Anti-TNFa)

Matrices listed below were spiked with certain level ofAnti-Tumor Necrosis Factor Alpha Antibody (Anti-TNFa) and the recovery rates were calculated by comparing the measured value to the expected amount of Anti-Tumor Necrosis Factor Alpha Antibody (Anti-TNFa) in samples.

MatrixRecovery range (%)Average(%)
serum(n=5)93-10196
EDTA plasma(n=5)81-10189
heparin plasma(n=5)97-105101

Precision of the ELISA Kit for Anti-Tumor Necrosis Factor Alpha Antibody (Anti-TNFa)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Anti-Tumor Necrosis Factor Alpha Antibody (Anti-TNFa) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Anti-Tumor Necrosis Factor Alpha Antibody (Anti-TNFa) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10%>Inter-Assay: CV<12%>

Linearity of the ELISA Kit for Anti-Tumor Necrosis Factor Alpha Antibody (Anti-TNFa)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Anti-Tumor Necrosis Factor Alpha Antibody (Anti-TNFa) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)80-93%80-102%80-101%78-90%
EDTA plasma(n=5)96-105%83-93%79-92%88-97%
heparin plasma(n=5)89-103%80-90%87-95%96-105%

Stability of the ELISA Kit for Anti-Tumor Necrosis Factor Alpha Antibody (Anti-TNFa)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the ELISA Kit for Anti-Tumor Necrosis Factor Alpha Antibody (Anti-TNFa)

1. Prepare all reagents, samples and standards;2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;4. Aspirate and wash 5 times;5. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;6. Add 50µL Stop Solution. Read at 450nm immediately.

Test principle of the ELISA Kit for Anti-Tumor Necrosis Factor Alpha Antibody (Anti-TNFa)

The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Anti-Tumor Necrosis Factor Alpha Antibody (Anti-TNFa) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Anti-Tumor Necrosis Factor Alpha Antibody (Anti-TNFa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

GIVEAWAYS

ELISA / CLIA Experiment Service

INCREMENT SERVICES

Single-component Reagents of Assay KitLysis Buffer Specific for ELISA / CLIAQuality Control of ELISA / CLIADisease Model Customized ServiceTGFB1 Activation ReagentReal Time PCR Experimental Service

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Catalog No.Organism species: Homo sapiens (Human) Applications (RESEARCH USE ONLY!)
APA133Hu01Active Tumor Necrosis Factor Alpha (TNFa)Cell culture; Activity Assays.
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PAA133Hu01Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa)WB; IHC; ICC; IP.
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GUESTBOOK
品牌介绍

Uscnk重组胃泌素(GT)

GAST;加油站; 胃泌素成分I;大胃泌素;胃泌素成分II; 胃泌素成分III

  • 产品编号

  • RPB224Mu01

  • 生物物种小家鼠(Mouse musculus)(鼠)同名,不同种。

  • 资源原核表达

  • 主办大肠杆菌

  • 内毒素水平每1µg <1.0EU(通过LAL方法确定)

  • 亚细胞定位秘密的

  • 预测分子量42.6kDa的

  • 准确的分子质量43kDa(差异分析请参见手册)

  • 残基和标签Net His和GST标签的Met1〜Gln101

  • 缓冲液配方20mM Tris,150mM NaCl,pH8.0,包含1mM EDTA,1mM DTT,0.01%SKL,5%海藻糖和Proclin300。

  • 特质冻干粉

  • 纯度> 90%

  • 等电点6.3

  • 应用领域积极控制;免疫原 SDS-PAGE;WB。
    如果需要蛋白质的生物活性,请检查活性蛋白质。

  • 下载使用说明书

  • 单位10微克为50μg 200μg的 1mg的 5毫克

  • 离岸价US $ 480



重组胃泌素(GT)的序列




重组胃泌素(GT)的用途

在20mM Tris,150mM NaCl(pH8.0)中复溶至0.1-1.0 mg / mL的浓度。不要涡旋。

重组胃泌素(GT)的存储

避免重复冷冻/解冻循环。在2-8°C下保存一个月。分装并在-80°C下存储12个月。

重组胃泌素(GT)的稳定性

热稳定性由损失率描述。损失率通过加速热降解试验确定,即将蛋白质在37°C下孵育48h,没有观察到明显的降解和沉淀。在适当的存储条件下,有效期内的丢失率小于5%。

礼物

聚丙烯酰胺凝胶电泳(PAGE)实验服务


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