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主营:分子类,蛋白类,抗体类,生化类试剂
℡ 4000-520-616
℡ 4000-520-616
Uscnk/CLIA Kit for Amphiregulin (AREG)/96T*100/SCA006Hu
产品编号:SCA006Hu
市  场 价:¥1176000.00
场      地:美国(厂家直采)
产品分类: 抗体类>二抗>抗牛>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$58800.00
品      牌: Uscnk
公      司:Uscnk
公司分类:
Uscnk/CLIA Kit for Amphiregulin (AREG)/96T*100/SCA006Hu
商品介绍

CLIA Kit for Amphiregulin (AREG)

AR; CRDGF; SDGF; Colorectum Cell-Derived Growth Factor; Schwannoma-Derived Growth Factor

  • Cytokine
  • Tumor immunity
  • Product No.SCA006Hu
  • Organism SpeciesHomo sapiens (Human) Same name, Different species.
    • All
    • Human
    • Mouse
    • Rat
    • Cavia
    • Rabbit
    • Simian
    • Caprine
    • Ovine
    • Equine
    • Bovine
    • Porcine
    • Gallus
    • Canine
    • Others
    • Multi-species
    • Pan-species
  • Test MethodDouble-antibody Sandwich
  • Assay Length2h, 40min
  • Detection Range1.37-1,000pg/mL
  • SensitivityThe minimum detectable dose of this kit is typically less than 0.59pg/mL.
  • Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • DownloadInstruction Manual
  • UOM48T96T96T*596T*1096T*100
  • FOBUS$ 588 For more details, please contact local distributors!US$ 840 For more details, please contact local distributors!US$ 3780 For more details, please contact local distributors!US$ 7140 For more details, please contact local distributors!US$ 58800 For more details, please contact local distributors!
  • CLIA Kit for Amphiregulin (AREG)Packages (Simulation)
  • CLIA Kit for Amphiregulin (AREG)Packages (Simulation)
  • CLIA Kit for Amphiregulin (AREG)Results demonstration
  • SCA006Hu.jpgTypical Standard Curve
  • CertificateISO9001: 2008, ISO13485: 2003 Registered

Specificity of the CLIA Kit for Amphiregulin (AREG)

This assay has high sensitivity and excellent specificity for detection of Amphiregulin (AREG).No significant cross-reactivity or interference between Amphiregulin (AREG) and analogues was observed.

Recovery of the CLIA Kit for Amphiregulin (AREG)

Matrices listed below were spiked with certain level of recombinant Amphiregulin (AREG) and the recovery rates were calculated by comparing the measured value to the expected amount of Amphiregulin (AREG) in samples.

MatrixRecovery range (%)Average(%)
serum(n=5)90-9894
EDTA plasma(n=5)79-9988
heparin plasma(n=5)98-105102

Precision of the CLIA Kit for Amphiregulin (AREG)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Amphiregulin (AREG) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Amphiregulin (AREG) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10%>Inter-Assay: CV<12%>

Linearity of the CLIA Kit for Amphiregulin (AREG)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Amphiregulin (AREG) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)90-101%93-104%93-101%95-103%
EDTA plasma(n=5)79-90%84-103%80-91%98-105%
heparin plasma(n=5)89-97%85-99%94-105%97-104%

Stability of the CLIA Kit for Amphiregulin (AREG)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the CLIA Kit for Amphiregulin (AREG)

1. Prepare all reagents, samples and standards;2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;4. Aspirate and wash 3 times;5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;6. Aspirate and wash 5 times;7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;8. Read RLU value immediately.

Test principle of the CLIA Kit for Amphiregulin (AREG)

The microplate provided in this kit has been pre-coated with an antibody specific to Amphiregulin (AREG). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Amphiregulin (AREG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Amphiregulin (AREG) level in the sample or standard.;

GIVEAWAYS

ELISA / CLIA Experiment Service

INCREMENT SERVICES

Single-component Reagents of Assay KitLysis Buffer Specific for ELISA / CLIAQuality Control of ELISA / CLIACLIA Kit Customized ServiceDisease Model Customized ServiceSerums Customized ServiceTGFB1 Activation ReagentReal Time PCR Experimental ServiceStreptavidin

Related products

Catalog No.Organism species: Homo sapiens (Human) Applications (RESEARCH USE ONLY!)
APA006Hu01Active Amphiregulin (AREG)Cell culture; Activity Assays.
RPA006Hu01Recombinant Amphiregulin (AREG)Positive Control; Immunogen; SDS-PAGE; WB.
PAA006Hu01Polyclonal Antibody to Amphiregulin (AREG)WB; IHC; ICC; IP.
MAA006Hu22Monoclonal Antibody to Amphiregulin (AREG)WB; IHC; ICC; IP.
MAA006Hu24Monoclonal Antibody to Amphiregulin (AREG)WB; IHC; ICC; IP.
MAA006Hu26Monoclonal Antibody to Amphiregulin (AREG)WB; IHC; ICC; IP.
MAA006Hu21Monoclonal Antibody to Amphiregulin (AREG)WB; IHC; ICC; IP.
LAA006Hu76Biotin-Linked Monoclonal Antibody to Amphiregulin (AREG)WB; IHC; ICC.
SEA006HuELISA Kit for Amphiregulin (AREG)Enzyme-linked immunosorbent assay for Antigen Detection.
SCA006HuCLIA Kit for Amphiregulin (AREG)Chemiluminescent immunoassay for Antigen Detection.
PSA006Hu01Antibody Pair for Amphiregulin (AREG)ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
KSA006Hu01ELISA Kit DIY Materials for Amphiregulin (AREG)Main materials for "Do It (ELISA Kit) Yourself".
GUESTBOOK
品牌介绍

Uscnk重组胃泌素(GT)

GAST;加油站; 胃泌素成分I;大胃泌素;胃泌素成分II; 胃泌素成分III

  • 产品编号

  • RPB224Mu01

  • 生物物种小家鼠(Mouse musculus)(鼠)同名,不同种。

  • 资源原核表达

  • 主办大肠杆菌

  • 内毒素水平每1µg <1.0EU(通过LAL方法确定)

  • 亚细胞定位秘密的

  • 预测分子量42.6kDa的

  • 准确的分子质量43kDa(差异分析请参见手册)

  • 残基和标签Net His和GST标签的Met1〜Gln101

  • 缓冲液配方20mM Tris,150mM NaCl,pH8.0,包含1mM EDTA,1mM DTT,0.01%SKL,5%海藻糖和Proclin300。

  • 特质冻干粉

  • 纯度> 90%

  • 等电点6.3

  • 应用领域积极控制;免疫原 SDS-PAGE;WB。
    如果需要蛋白质的生物活性,请检查活性蛋白质。

  • 下载使用说明书

  • 单位10微克为50μg 200μg的 1mg的 5毫克

  • 离岸价US $ 480



重组胃泌素(GT)的序列




重组胃泌素(GT)的用途

在20mM Tris,150mM NaCl(pH8.0)中复溶至0.1-1.0 mg / mL的浓度。不要涡旋。

重组胃泌素(GT)的存储

避免重复冷冻/解冻循环。在2-8°C下保存一个月。分装并在-80°C下存储12个月。

重组胃泌素(GT)的稳定性

热稳定性由损失率描述。损失率通过加速热降解试验确定,即将蛋白质在37°C下孵育48h,没有观察到明显的降解和沉淀。在适当的存储条件下,有效期内的丢失率小于5%。

礼物

聚丙烯酰胺凝胶电泳(PAGE)实验服务


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