CLIA Kit for Interferon Gamma (IFNg)
IFN-G; IFG; IFI; INFr; IFN, Immune Interferon
- Product No.SCA049Ra
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Caprine
- Ovine
- Equine
- Bovine
- Porcine
- Gallus
- Canine
- Others
- Multi-species
- Pan-species
- Test MethodDouble-antibody Sandwich
- Assay Length2h, 40min
- Detection Range1.37-1,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.49pg/mL.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- DownloadInstruction Manual
- UOM48T96T96T*596T*1096T*100
- FOBUS$ 638 For more details, please contact local distributors!US$ 912 For more details, please contact local distributors!US$ 4104 For more details, please contact local distributors!US$ 7752 For more details, please contact local distributors!US$ 63840 For more details, please contact local distributors!
Packages (Simulation)- Packages (Simulation)
- Results demonstration
- Typical Standard Curve
- ISO9001: 2008, ISO13485: 2003 Registered
Specificity of the CLIA Kit for Interferon Gamma (IFNg)
This assay has high sensitivity and excellent specificity for detection of Interferon Gamma (IFNg).No significant cross-reactivity or interference between Interferon Gamma (IFNg) and analogues was observed.
Recovery of the CLIA Kit for Interferon Gamma (IFNg)
Matrices listed below were spiked with certain level of recombinant Interferon Gamma (IFNg) and the recovery rates were calculated by comparing the measured value to the expected amount of Interferon Gamma (IFNg) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 95-103 | 101 |
| EDTA plasma(n=5) | 91-99 | 95 |
| heparin plasma(n=5) | 79-89 | 84 |
Precision of the CLIA Kit for Interferon Gamma (IFNg)
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interferon Gamma (IFNg) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interferon Gamma (IFNg) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10%>Inter-Assay: CV<12%>
Linearity of the CLIA Kit for Interferon Gamma (IFNg)
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Interferon Gamma (IFNg) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 79-99% | 90-98% | 80-96% | 96-103% |
| EDTA plasma(n=5) | 92-99% | 87-101% | 96-105% | 79-98% |
| heparin plasma(n=5) | 83-104% | 94-104% | 81-90% | 89-101% |
Stability of the CLIA Kit for Interferon Gamma (IFNg)
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary of the CLIA Kit for Interferon Gamma (IFNg)
1. Prepare all reagents, samples and standards;2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;4. Aspirate and wash 3 times;5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;6. Aspirate and wash 5 times;7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;8. Read RLU value immediately.
Test principle of the CLIA Kit for Interferon Gamma (IFNg)
The microplate provided in this kit has been pre-coated with an antibody specific to Interferon Gamma (IFNg). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Interferon Gamma (IFNg). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Interferon Gamma (IFNg) level in the sample or standard.;
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ELISA / CLIA Experiment Service
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Single-component Reagents of Assay KitLysis Buffer Specific for ELISA / CLIAQuality Control of ELISA / CLIACLIA Kit Customized ServiceDisease Model Customized ServiceSerums Customized ServiceTGFB1 Activation ReagentReal Time PCR Experimental ServiceStreptavidin
Related products
| Catalog No. | Organism species: Rattus norvegicus (Rat) | Applications (RESEARCH USE ONLY!) |
| APA049Ra01 | Active Interferon Gamma (IFNg) | Cell culture; Activity Assays. |
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| APA049Ra61 | Active Interferon Gamma (IFNg) | Cell culture; Activity Assays. |
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| LAA049Ra81 | FITC-Linked Polyclonal Antibody to Interferon Gamma (IFNg) | WB; IHC; ICC; IF. |
| LAA049Ra71 | Biotin-Linked Polyclonal Antibody to Interferon Gamma (IFNg) | WB; IHC; ICC. |
| MAA049Ra21 | Monoclonal Antibody to Interferon Gamma (IFNg) | WB; IHC; ICC; IP. |
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| SCA049Ra | CLIA Kit for Interferon Gamma (IFNg) | Chemiluminescent immunoassay for Antigen Detection. |
| KSA049Ra01 | ELISA Kit DIY Materials for Interferon Gamma (IFNg) | Main materials for "Do It (ELISA Kit) Yourself". |
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Uscnk重组胃泌素（GT）
GAST；加油站; 胃泌素成分I；大胃泌素；胃泌素成分II; 胃泌素成分III
重组胃泌素（GT）的序列
重组胃泌素（GT）的用途
在20mM Tris，150mM NaCl（pH8.0）中复溶至0.1-1.0 mg / mL的浓度。不要涡旋。
重组胃泌素（GT）的存储
避免重复冷冻/解冻循环。在2-8°C下保存一个月。分装并在-80°C下存储12个月。
重组胃泌素（GT）的稳定性
热稳定性由损失率描述。损失率通过加速热降解试验确定，即将蛋白质在37°C下孵育48h，没有观察到明显的降解和沉淀。在适当的存储条件下，有效期内的丢失率小于5％。
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