CLIA Kit for Interleukin 10 (IL10)
CSIF; IL10A; TGIF; Cytokine Synthesis Inhibitory Factor
- Product No.SCA056Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Caprine
- Ovine
- Equine
- Bovine
- Porcine
- Gallus
- Canine
- Others
- Multi-species
- Pan-species
- Test MethodDouble-antibody Sandwich
- Assay Length2h, 40min
- Detection Range1.37-1,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.54pg/mL.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- DownloadInstruction Manual
- UOM48T96T96T*596T*1096T*100
- FOBUS$ 605 For more details, please contact local distributors!US$ 864 For more details, please contact local distributors!US$ 3888 For more details, please contact local distributors!US$ 7344 For more details, please contact local distributors!US$ 60480 For more details, please contact local distributors!
Packages (Simulation)- Packages (Simulation)
- Results demonstration
- Typical Standard Curve
- ISO9001: 2008, ISO13485: 2003 Registered
Specificity of the CLIA Kit for Interleukin 10 (IL10)
This assay has high sensitivity and excellent specificity for detection of Interleukin 10 (IL10).No significant cross-reactivity or interference between Interleukin 10 (IL10) and analogues was observed.
Recovery of the CLIA Kit for Interleukin 10 (IL10)
Matrices listed below were spiked with certain level of recombinant Interleukin 10 (IL10) and the recovery rates were calculated by comparing the measured value to the expected amount of Interleukin 10 (IL10) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 84-101 | 94 |
| EDTA plasma(n=5) | 78-93 | 87 |
| heparin plasma(n=5) | 86-101 | 96 |
Precision of the CLIA Kit for Interleukin 10 (IL10)
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 10 (IL10) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 10 (IL10) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10%>Inter-Assay: CV<12%>
Linearity of the CLIA Kit for Interleukin 10 (IL10)
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Interleukin 10 (IL10) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 89-105% | 93-101% | 85-104% | 89-103% |
| EDTA plasma(n=5) | 85-98% | 89-96% | 82-99% | 88-96% |
| heparin plasma(n=5) | 95-103% | 83-92% | 97-104% | 88-96% |
Stability of the CLIA Kit for Interleukin 10 (IL10)
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary of the CLIA Kit for Interleukin 10 (IL10)
1. Prepare all reagents, samples and standards;2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;4. Aspirate and wash 3 times;5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;6. Aspirate and wash 5 times;7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;8. Read RLU value immediately.
Test principle of the CLIA Kit for Interleukin 10 (IL10)
The microplate provided in this kit has been pre-coated with an antibody specific to Interleukin 10 (IL10). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Interleukin 10 (IL10). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Interleukin 10 (IL10) level in the sample or standard.;
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Single-component Reagents of Assay KitLysis Buffer Specific for ELISA / CLIAQuality Control of ELISA / CLIACLIA Kit Customized ServiceDisease Model Customized ServiceSerums Customized ServiceTGFB1 Activation ReagentReal Time PCR Experimental ServiceStreptavidin
Related products
| Catalog No. | Organism species: Mus musculus (Mouse) | Applications (RESEARCH USE ONLY!) |
| EPA056Mu61 | Eukaryotic Interleukin 10 (IL10) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA056Mu01 | Recombinant Interleukin 10 (IL10) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA056Mu02 | Recombinant Interleukin 10 (IL10) | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA056Mu61 | Active Interleukin 10 (IL10) | Cell culture; Activity Assays. |
| PAA056Mu01 | Polyclonal Antibody to Interleukin 10 (IL10) | WB; IHC; ICC; IP. |
| PAA056Mu02 | Polyclonal Antibody to Interleukin 10 (IL10) | WB; IHC; ICC; IP. |
| LAA056Mu71 | Biotin-Linked Polyclonal Antibody to Interleukin 10 (IL10) | WB; IHC; ICC. |
| LAA056Mu81 | FITC-Linked Polyclonal Antibody to Interleukin 10 (IL10) | WB; IHC; ICC; IF. |
| MAA056Mu21 | Monoclonal Antibody to Interleukin 10 (IL10) | WB; IHC; ICC; IP. |
| HEA056Mu | High Sensitive ELISA Kit for Interleukin 10 (IL10) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SEA056Mu | ELISA Kit for Interleukin 10 (IL10) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MEA056Mu | Mini Samples ELISA Kit for Interleukin 10 (IL10) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA056Mu | CLIA Kit for Interleukin 10 (IL10) | Chemiluminescent immunoassay for Antigen Detection. |
Uscnk重组胃泌素（GT）
GAST；加油站; 胃泌素成分I；大胃泌素；胃泌素成分II; 胃泌素成分III
重组胃泌素（GT）的序列
重组胃泌素（GT）的用途
在20mM Tris，150mM NaCl（pH8.0）中复溶至0.1-1.0 mg / mL的浓度。不要涡旋。
重组胃泌素（GT）的存储
避免重复冷冻/解冻循环。在2-8°C下保存一个月。分装并在-80°C下存储12个月。
重组胃泌素（GT）的稳定性
热稳定性由损失率描述。损失率通过加速热降解试验确定，即将蛋白质在37°C下孵育48h，没有观察到明显的降解和沉淀。在适当的存储条件下，有效期内的丢失率小于5％。
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