ELISA Kit DIY Materials for Tumor Necrosis Factor Alpha (TNFa)
DIF; TNF-A; TNFSF2; Cachectin; Tumor Necrosis Factor Ligand Superfamily Member 2
- Product No.KSA133Ov01
- Organism SpeciesOvis aries; Ovine (Sheep) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Caprine
- Ovine
- Equine
- Bovine
- Porcine
- Gallus
- Canine
- Others
- Multi-species
- Pan-species
- Reagent ContentsCapture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable Sampleserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range3.12-200pg/mL
- Applicable Sensitivity1.21pg/mL
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
- Downloadn/a
- UOM96T*596T*1096T*2096T*5096T*100
- FOBUS$ 1458 For more details, please contact local distributors!US$ 2430 For more details, please contact local distributors!US$ 4374 For more details, please contact local distributors!US$ 8505 For more details, please contact local distributors!US$ 14580 For more details, please contact local distributors!
Packages (Simulation)- Packages (Simulation)
- ISO9001: 2008, ISO13485: 2003 Registered
SPECIFITY of the ELISA Kit DIY Materials for Tumor Necrosis Factor Alpha (TNFa)
The Abs in the kit have high sensitivity and excellent specificity for detection of Tumor Necrosis Factor Alpha (TNFa). No significant cross-reactivity or interference between Tumor Necrosis Factor Alpha (TNFa) and analogues was observed.
USAGE of the ELISA Kit DIY Materials for Tumor Necrosis Factor Alpha (TNFa)
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.2. Aspirate and wash 1 time.3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
STORAGE of the ELISA Kit DIY Materials for Tumor Necrosis Factor Alpha (TNFa)
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
SUPPORT PACK of the ELISA Kit DIY Materials for Tumor Necrosis Factor Alpha (TNFa)
GIVEAWAYS
ELISA / CLIA Experiment Service
INCREMENT SERVICES
CLIA Kit Customized ServiceELISA Kit Customized ServiceAssay Kit DIY Support Pack 1Assay Kit DIY Support Pack 2Standard DiluentCoating BufferDiluent Buffer for AntibodyAssay Kit DIY Support Pack 3Assay Kit DIY Support Pack 4Real Time PCR Experimental Service
Related products
| Catalog No. | Organism species: Ovis aries; Ovine (Sheep) | Applications (RESEARCH USE ONLY!) |
| RPA133Ov01 | Recombinant Tumor Necrosis Factor Alpha (TNFa) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA133Ov01 | Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa) | WB; IHC; ICC; IP. |
| LAA133Ov71 | Biotin-Linked Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa) | WB; IHC; ICC. |
| MAA133Ov21 | Monoclonal Antibody to Tumor Necrosis Factor Alpha (TNFa) | WB; IHC; ICC; IP. |
| SEA133Ov | ELISA Kit for Tumor Necrosis Factor Alpha (TNFa) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| KSA133Ov01 | ELISA Kit DIY Materials for Tumor Necrosis Factor Alpha (TNFa) | Main materials for "Do It (ELISA Kit) Yourself". |
Uscnk重组胃泌素（GT）
GAST；加油站; 胃泌素成分I；大胃泌素；胃泌素成分II; 胃泌素成分III
重组胃泌素（GT）的序列
重组胃泌素（GT）的用途
在20mM Tris，150mM NaCl（pH8.0）中复溶至0.1-1.0 mg / mL的浓度。不要涡旋。
重组胃泌素（GT）的存储
避免重复冷冻/解冻循环。在2-8°C下保存一个月。分装并在-80°C下存储12个月。
重组胃泌素（GT）的稳定性
热稳定性由损失率描述。损失率通过加速热降解试验确定，即将蛋白质在37°C下孵育48h，没有观察到明显的降解和沉淀。在适当的存储条件下，有效期内的丢失率小于5％。
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