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主营:分子类,蛋白类,抗体类,生化类试剂
℡ 4000-520-616
℡ 4000-520-616
Uscnk/CLIA Kit for Fibroblast Growth Factor 18 (FGF18)/96T*100/CCC907Hu
产品编号:CCC907Hu
市  场 价:¥1142400.00
场      地:美国(厂家直采)
产品分类: 蛋白类>多肽>多肽合成>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$57120.00
品      牌: Uscnk
公      司:Uscnk
公司分类:
Uscnk/CLIA Kit for Fibroblast Growth Factor 18 (FGF18)/96T*100/CCC907Hu
商品介绍

CLIA Kit for Fibroblast Growth Factor 18 (FGF18)

ZFGF5

  • Cytokine
  • Product No.CCC907Hu
  • Organism SpeciesHomo sapiens (Human) Same name, Different species.
    • All
    • Human
    • Mouse
    • Rat
    • Cavia
    • Rabbit
    • Simian
    • Caprine
    • Ovine
    • Equine
    • Bovine
    • Porcine
    • Gallus
    • Canine
    • Others
    • Multi-species
    • Pan-species
  • Test MethodCompetitive Inhibition
  • Assay Length2h
  • Detection Range31.2-500pg/mL
  • SensitivityThe minimum detectable dose of this kit is typically less than 13.3pg/mL.
  • Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • DownloadInstruction Manual
  • UOM48T96T96T*596T*1096T*100
  • FOBUS$ 571 For more details, please contact local distributors!US$ 816 For more details, please contact local distributors!US$ 3672 For more details, please contact local distributors!US$ 6936 For more details, please contact local distributors!US$ 57120 For more details, please contact local distributors!
  • CLIA Kit for Fibroblast Growth Factor 18 (FGF18)Packages (Simulation)
  • CLIA Kit for Fibroblast Growth Factor 18 (FGF18)Packages (Simulation)
  • CLIA Kit for Fibroblast Growth Factor 18 (FGF18)Results demonstration
  • CCC907Hu.jpgTypical Standard Curve
  • CertificateISO9001: 2008, ISO13485: 2003 Registered

Specificity of the CLIA Kit for Fibroblast Growth Factor 18 (FGF18)

This assay has high sensitivity and excellent specificity for detection of Fibroblast Growth Factor 18 (FGF18).No significant cross-reactivity or interference between Fibroblast Growth Factor 18 (FGF18) and analogues was observed.

Recovery of the CLIA Kit for Fibroblast Growth Factor 18 (FGF18)

Matrices listed below were spiked with certain level of recombinant Fibroblast Growth Factor 18 (FGF18) and the recovery rates were calculated by comparing the measured value to the expected amount of Fibroblast Growth Factor 18 (FGF18) in samples.

MatrixRecovery range (%)Average(%)
serum(n=5)97-105101
EDTA plasma(n=5)79-9582
heparin plasma(n=5)78-8982

Precision of the CLIA Kit for Fibroblast Growth Factor 18 (FGF18)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibroblast Growth Factor 18 (FGF18) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibroblast Growth Factor 18 (FGF18) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10%>Inter-Assay: CV<12%>

Linearity of the CLIA Kit for Fibroblast Growth Factor 18 (FGF18)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Fibroblast Growth Factor 18 (FGF18) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)93-103%95-102%93-101%87-95%
EDTA plasma(n=5)79-99%93-104%79-95%85-92%
heparin plasma(n=5)78-95%96-104%98-105%93-101%

Stability of the CLIA Kit for Fibroblast Growth Factor 18 (FGF18)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the CLIA Kit for Fibroblast Growth Factor 18 (FGF18)

1. Prepare all reagents, samples and standards;2. Add 50µL standard or sample to each well.And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C;3. Aspirate and wash 3 times;4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;5. Aspirate and wash 5 times;6. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;7. Read RLU value immediately.

Test principle of the CLIA Kit for Fibroblast Growth Factor 18 (FGF18)

The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Fibroblast Growth Factor 18 (FGF18). A competitive inhibition reaction is launched between biotin labeled Fibroblast Growth Factor 18 (FGF18) and unlabeled Fibroblast Growth Factor 18 (FGF18) (Standards or samples) with the pre-coated antibody specific to Fibroblast Growth Factor 18 (FGF18). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Fibroblast Growth Factor 18 (FGF18) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Fibroblast Growth Factor 18 (FGF18) level in the sample or standard.

GIVEAWAYS

ELISA / CLIA Experiment Service

INCREMENT SERVICES

Single-component Reagents of Assay KitLysis Buffer Specific for ELISA / CLIAQuality Control of ELISA / CLIACLIA Kit Customized ServiceDisease Model Customized ServiceSerums Customized ServiceTGFB1 Activation ReagentReal Time PCR Experimental Service

Related products

Catalog No.Organism species: Homo sapiens (Human) Applications (RESEARCH USE ONLY!)
RPC907Hu01Recombinant Fibroblast Growth Factor 18 (FGF18)Positive Control; Immunogen; SDS-PAGE; WB.
APC907Hu01Active Fibroblast Growth Factor 18 (FGF18)Cell culture; Activity Assays.
PAC907Hu01Polyclonal Antibody to Fibroblast Growth Factor 18 (FGF18)WB; IHC; ICC; IP.
MAC907Hu22Monoclonal Antibody to Fibroblast Growth Factor 18 (FGF18)WB; IHC; ICC; IP.
SEC907HuELISA Kit for Fibroblast Growth Factor 18 (FGF18)Enzyme-linked immunosorbent assay for Antigen Detection.
CEC907HuELISA Kit for Fibroblast Growth Factor 18 (FGF18)Enzyme-linked immunosorbent assay for Antigen Detection.
SCC907HuCLIA Kit for Fibroblast Growth Factor 18 (FGF18)Chemiluminescent immunoassay for Antigen Detection.
CCC907HuCLIA Kit for Fibroblast Growth Factor 18 (FGF18)Chemiluminescent immunoassay for Antigen Detection.
GUESTBOOK
品牌介绍

Uscnk重组胃泌素(GT)

GAST;加油站; 胃泌素成分I;大胃泌素;胃泌素成分II; 胃泌素成分III

  • 产品编号

  • RPB224Mu01

  • 生物物种小家鼠(Mouse musculus)(鼠)同名,不同种。

  • 资源原核表达

  • 主办大肠杆菌

  • 内毒素水平每1µg <1.0EU(通过LAL方法确定)

  • 亚细胞定位秘密的

  • 预测分子量42.6kDa的

  • 准确的分子质量43kDa(差异分析请参见手册)

  • 残基和标签Net His和GST标签的Met1〜Gln101

  • 缓冲液配方20mM Tris,150mM NaCl,pH8.0,包含1mM EDTA,1mM DTT,0.01%SKL,5%海藻糖和Proclin300。

  • 特质冻干粉

  • 纯度> 90%

  • 等电点6.3

  • 应用领域积极控制;免疫原 SDS-PAGE;WB。
    如果需要蛋白质的生物活性,请检查活性蛋白质。

  • 下载使用说明书

  • 单位10微克为50μg 200μg的 1mg的 5毫克

  • 离岸价US $ 480



重组胃泌素(GT)的序列




重组胃泌素(GT)的用途

在20mM Tris,150mM NaCl(pH8.0)中复溶至0.1-1.0 mg / mL的浓度。不要涡旋。

重组胃泌素(GT)的存储

避免重复冷冻/解冻循环。在2-8°C下保存一个月。分装并在-80°C下存储12个月。

重组胃泌素(GT)的稳定性

热稳定性由损失率描述。损失率通过加速热降解试验确定,即将蛋白质在37°C下孵育48h,没有观察到明显的降解和沉淀。在适当的存储条件下,有效期内的丢失率小于5%。

礼物

聚丙烯酰胺凝胶电泳(PAGE)实验服务


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